Journal: Cell Death & Disease

Article Title: A novel lncRNA n384546 promotes thyroid papillary cancer progression and metastasis by acting as a competing endogenous RNA of miR-145-5p to regulate AKT3

doi: 10.1038/s41419-019-1637-7

Figure Lengend Snippet: a Validation of Gapmer-n384546 knockdown efficiency in B-CPAP and KTC-1 cells was determined by qRT-PCR. b CCK-8 proliferation assay in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. c Colony formation assay in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. d EdU proliferation assay in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. e Flow cytometric analysis of apoptosis in Scrambled Gapmer or Gapmer-n384546 transfected B-CPAP and KTC-1 cells. f Tumor size and tumor weight of nude mice was measured and analyzed. g Tumor volume curves of nude mice injected with sh-control and sh-n384546 B-CPAP cells were analyzed. h n384546 expression in tumors collected from nude mice was determined by qRT-PCR. i Immunohistochemical staining of Bcl-2, caspase9 and Ki-67 was used to assess proliferation and apoptosis (400×). Data represent the mean ± SEM of three separate experiments. All experiments were repeated at least three times. * p < 0.05, ** p < 0.01 in independent Student’s t test

Article Snippet: Cell proliferation and viability were analyzed using CCK8 (Cell Counting Kit-8, Dojindo, Japan) following the protocol provided by the manufacturer.

Techniques: Biomarker Discovery, Knockdown, Quantitative RT-PCR, CCK-8 Assay, Proliferation Assay, Transfection, Colony Assay, Injection, Control, Expressing, Immunohistochemical staining, Staining

Journal: Cell Death & Disease

Article Title: A novel lncRNA n384546 promotes thyroid papillary cancer progression and metastasis by acting as a competing endogenous RNA of miR-145-5p to regulate AKT3

doi: 10.1038/s41419-019-1637-7

Figure Lengend Snippet: a CCK-8 proliferation assay, b EdU proliferation assay, c Flow cytometric analysis of apoptosis, d Transwell invasion assay, e Transwell migration assay, and f Wound healing assay were performed in B-CPAP and KTC-1 cells transfected with Scrambled Gapmer, anti-miR-145, mimic-miR-145, Gapmer-n384546, Gapmer-n384546 + anti-miR-145, Gapmer-n384546 + mimic-miR-145. g The expression of proteins in B-CPAP cells transfected with Scrambled Gapmer, anti-miR-145, mimic-miR-145, Gapmer-n384546, Gapmer-n384546 + anti-miR-145, Gapmer-n384546 + mimic-miR-145 was determined by western blot. Data represent the mean ± SEM of three separate experiments. All experiments were repeated at least three times. * p < 0.05, ** p < 0.01 in independent Student’s t test ( a – f )

Article Snippet: Cell proliferation and viability were analyzed using CCK8 (Cell Counting Kit-8, Dojindo, Japan) following the protocol provided by the manufacturer.

Techniques: CCK-8 Assay, Proliferation Assay, Transwell Invasion Assay, Transwell Migration Assay, Wound Healing Assay, Transfection, Expressing, Western Blot

Journal: Journal of Oncology

Article Title: The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma

doi: 10.1155/2021/9958483

Figure Lengend Snippet: The impact of PC4 on the malignant phenotypes of lung adenocarcinoma cells. (a), (b) Western blot assay revealed the protein levels of PC4 after LUAD cells were infected with PC4-knockdown or PC4-overexpression lentivirus. (c) The apoptosis of PC4-knockdown or PC4-overexpression LUAD cells was evaluated by flow cytometry. (d) The growth of PC4-knockdown or PC4-overexpression LUAD cells was analyzed via CCK-8 experiments. (e) The migration of LUAD cells following PC4 knockdown or overexpression was detected via Transwell assay. ∗ p < 0.05.

Article Snippet: The CCK-8 test was accomplished using the Cell Counting Kit-8 (CCK-8) (Bioss, China) as we previously described [ ].

Techniques: Western Blot, Infection, Over Expression, Flow Cytometry, CCK-8 Assay, Migration, Transwell Assay

Journal: Journal of Oncology

Article Title: The Human Positive Cofactor 4 is a Promising Chemotherapeutic Target in Lung Adenocarcinoma

doi: 10.1155/2021/9958483

Figure Lengend Snippet: PC4 decreases cisplatin's cytotoxic effects on lung adenocarcinoma cells in vitro . (a) PC4-knockdown or PC4-overexpression LUAD cells underwent 24-hour treatment with 20 μ M cisplatin. The expression of apoptotic proteins was evaluated. (b) PC4-knockdown or PC4-overexpression LUAD cells were administrated with 20 µ M cisplatin for 96 hours. The CCK-8 assay was performed every 24 hours. (c), (d) PC4-knockdown or PC4-overexpression LUAD cells underwent 24-hour treatment with 0, 20, and 30 µ M cisplatin. Cell apoptosis was measured and analyzed by flow cytometry. ∗ p < 0.05.

Article Snippet: The CCK-8 test was accomplished using the Cell Counting Kit-8 (CCK-8) (Bioss, China) as we previously described [ ].

Techniques: In Vitro, Over Expression, Expressing, CCK-8 Assay, Flow Cytometry

Journal: Stem Cells International

Article Title: AT7867 Inhibits the Growth of Colorectal Cancer Stem-Like Cells and Stemness by Regulating the Stem Cell Maintenance Factor Ascl2 and Akt Signaling

doi: 10.1155/2023/4199052

Figure Lengend Snippet: AT7867 inhibits the growth and stemness of CSCs. (a, b) By being cocultured with AT7867 for 1 to 3 days, cell viability was measured by performing CCK-8 assay. (c, d) Representative morphology after AT7867 treatment (sphere formation assay) or pretreatment (sphere recovery assay); Scale bar 200 μ m. (e, f) Oct4, Nanog, CD133, and CD44 protein levels were measured by performing Western blot. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 vs. the control.

Article Snippet: After incubating at 37°C for 24, 48, and 72 hours, CCK8 reagent (APExBIO, China) was added and the absorbance at 450 nm was measured after incubation for 1 hour.

Techniques: CCK-8 Assay, Tube Formation Assay, Western Blot, Control

Journal: Biomolecules

Article Title: Cytocompatibility of Modified Silk Fibroin with Glycidyl Methacrylate for Tissue Engineering and Biomedical Applications

doi: 10.3390/biom11010035

Figure Lengend Snippet: Cell proliferation and viability assay with NIH 3T3 on each dialysis period of modified silk fibroin with glycidyl methacrylate (Silk-GMA). ( A ). CCK 8 assay for cell proliferation rate gradually increased following dialysis period days 0, 1, 2, 4, and 7. **** p < 0.0001, *** p < 0.001, and ns = no significant. ( B ). NIH 3T3 cells were exposed with 0.1 g/mL of each dialyzed Silk-GMA sample for 72 h. NIH 3T3 cells were detected with Live & Dead assay with indication of calcein AM (live cells, green fluorescent protein) and ethidium homodimer-1 (dead cells, red fluorescent protein), separately. The confocal microscopic images showed that dead cells decreased, and then live cells proliferated gradually depending on dialysis periods (scale bar = 100 µm).

Article Snippet: Live & Dead assay kit and FITC Annexin V/Dead cell apoptosis kit were purchased from Invitrogen (Waltham, MA, USA), and the Cell Counting Kit-8 assay (CCK-8) was obtained from EnzoBiochem (New York, NY, USA).

Techniques: Viability Assay, Modification, CCK-8 Assay, Live Dead Assay